Afleveringen
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Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments.
Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system.
Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice.
Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy.
Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen.
In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper.
These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers.
Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art.
Tune in to discover how you can turn your passion for science and art into a fulfilling career.
Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Zijn er afleveringen die ontbreken?
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Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In.
Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching.
Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels.
Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training.
Watch the full presentation here: https://microscopyfocus.com/five-color-sted/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice.
Learn how to tackle issues like:
• Career disruption
• Reason for choosing your place of work
• Track record relative to opportunity
• Consumer involvementThis episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant!
Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com
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In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores.
But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging.
Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags.
These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies.
Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/ -
Stress is a universal challenge, and scientists face unique pressures such as impostor syndrome, the demand for positive results, and career uncertainty. These stressors can be overwhelming and often feel beyond our control.
In this episode of Listen In, we explore the various stresses that scientists encounter in their daily lab life and discuss effective strategies to manage them. Learn how to prevent burnout, anxiety, and depression by addressing issues like perfectionism and impostor syndrome. Our expert shares personal insights and practical advice on cultivating a resilient and healthier growth mindset.
Hit play to discover that you're not alone in feeling overwhelmed and that with the right mindset, you can navigate the pressures of a scientific career more effectively.
Watch the full presentation here: https://events.bitesizebio.com/stress-in-the-lab-common-pitfalls-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions.
Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions.
Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.
Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.
Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com
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Single cell sequencing samples must be prepared to the highest possible standard.
Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing.
In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample.
You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist.
Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Join Kate Christian as she demystifies the process of effectively communicating your research in the latest episode of Listen In.
She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements.
As well as learning how to work out your h-index, you will learn how to describe the impact of your papers.
Watch the full presentation here: https://events.bitesizebio.com/how-to-effectively-communicate-your-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Precise control of cellular communication and signaling is crucial for every physiological system, with G protein-coupled receptors (GPCRs, the largest family of signaling receptors) playing a central role as essential molecular sensors of a cell’s environment.
Because of their diverse physiological and pathophysiological roles, GPCRs are also major drug targets.
Our understanding of GPCR signaling has evolved from single receptors at the cell surface activating specific heterotrimeric G protein pathways, to an increasingly complex receptor signaling system. However, the complexity of GPCR signaling is translated to specific cellular and physiological responses is still unclear.
In this episode, we explore some of the latest endeavors to uncover GPCR signaling mechanisms through a wide range of imaging applications, from analyzing single molecules to the identification of new subcellular signal platforms.
Watch the full presentation here: https://microscopyfocus.com/imaging-gpcr-activity-unravelling-mechanisms-from-molecules-to-morphology/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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As a researcher, one of the most crucial skills to develop is the ability to perform an efficient yet thorough review of manuscripts.
Manuscript reviewing requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify and handle tricky issues such as image and data manipulation.
In this episode of Listen In, Dr. Johanna Ahlskog discusses the essential skills all reviewers and researchers need, emphasizes the critical importance of the review process, and offers general considerations when reviewing articles.
Watch the full presentation here: https://events.bitesizebio.com/critical-analysis-of-research-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution.
Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal and spatial resolution capabilities.
Imaging complex cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in live specimens are emerging avenues to shed light on biological processes in their native physiological context.
With the advent of STED, researchers have realized the visualization of intracellular structures at the nanoscale. Tune in to see how STED is enabling unprecedented insights into cellular behavior, interactions, and function.
Watch the full presentation here: https://microscopyfocus.com/new-tausted-tools-for-gentle-live-imaging-at-nanoscale-resolution/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Your research is important, but it can be hard to explain to others why it's important.
In this episode of Listen In, you'll learn to find the ideal hook for your science story.
This trick, borrowed from journalism, involves taking a step back and thinking about all the aspects of your research and where they might overlap with other topics that attract interest from other people.
Knowing how your research connects with other areas is useful when writing grant proposals or giving public talks about your work because it helps you write or speak persuasively.
Watch the full presentation here: https://events.bitesizebio.com/finding-the-ideal-hook-for-your/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Contamination poses a significant risk in cell culture, jeopardizing research outcomes and incurring costly setbacks.
This episode of Listen In gives you valuable insights into often overlooked areas of your filtration processes and will help you safeguard your sensitive cell cultures.
Explore the critical importance of sterile filtration in diverse applications leveraging Millipore® filtration technology.
Hear about the alarming consequences of suboptimal filters in sensitive media composition, highlighting the financial and time investments at stake.
Plus, discover how the use of Millipore® filters as the gold standard in both non-sterile and sterile filtration can mitigate contamination risks and uphold the integrity of your cultures.
Our expert guest showcases the superior performance of Millipore® membranes and explains their advanced features that enable efficient particle retention and exceptional flow characteristics.
Watch the full presentation here: https://events.bitesizebio.com/culture-media-preparation/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Despite the growing number of new technologies and automated instruments, routine liquid handling is still a manual process in many labs. The trend towards automation is gaining importance, not only with complex applications, such as NGS library preparation but also with simple yet repetitive liquid handling steps where automated pipetting makes sense.
A variety of automated liquid handling systems—suiting all budgets and workloads—are now available. These systems are designed to improve consistency and efficiency by eliminating both person-to-person and day-to-day variability.
But how to get started with automation? When does it make sense to automate workflows and methods, and isn't it super complicated to implement an automated liquid handler in a lab?
Get answers to these questions in this episode of Listen In!
Watch the full presentation here: https://events.bitesizebio.com/automate-your-pipetting-unleash/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Inspired by mutations associated with neurodevelopmental disorders, Prof. Dr. Silvia Cappello’s group at the Ludwig Maximilian University of Munich aims to understand the role of extracellular mechanisms essential for the correct development of the human brain.
Their research focuses on intrinsic and extrinsic mechanisms contributing to the formation of the brain by examining mutations in genes influencing cell-cell contacts, the extracellular matrix, and the secretion of vesicles.
In this episode of Listen In, Silvia presents the latest research that makes an important contribution to our understanding of cell non-autonomous mechanisms in the development of neurodevelopmental disorders.
She also explains how imaging workflows, using the Mica Imaging Microhub alongside confocal and super-resolution systems, support the group’s research.
Watch the full presentation here: https://microscopyfocus.com/cellular-crosstalk-in-neurodevelopmental-disorders/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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In this episode of Listen In, master your PCR set-up for unparalleled results.
Check out this enlightening discussion of the crucial factors that influence the outcome of PCR experiments and discover the parameters you can optimize for better and more consistent PCR results.
You will get reliable advice directly from PCR experts at Eppendorf on key topics, including the core features of thermal cyclers and the importance of good pipetting techniques to minimize evaporation.
This episode also features helpful insights into ideal reaction set-up, primer design, template DNA selection, and PCR optimization.
Watch the full presentation here: https://events.bitesizebio.com/end-point-pcr-set-up-and/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Improving open science and research reproducibility is both a technical and a social challenge.
But how do we do it?
One way is to design, conduct, and report research that can maximize the impact of our individual and collective investment in research.
Plus, changing the research culture to prioritize 'getting it right' over 'getting it published' promotes transparency and reproducibility without losing the engine of innovation and discovery that drives scientific inquiry.
In this episode of Listen In, get an overview of the fundamental challenges that reduce the credibility and reproducibility of research, how open science practices address these challenges, how you can incorporate them into your research, and how you can help promote a change in the research culture.
Watch the full presentation here: https://events.bitesizebio.com/approaches-for-more-transparent/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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For many researchers, modern science has become about getting results rather than asking questions. Results mean publications, and publications mean more funding.
However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true or false, and objectively interpreting the results.
This episode of Listen In explores the definition of the scientific method and how to apply it to your research.
Get a handle on the scientific method with a clear definition, and learn how to apply it to your research using real-life examples of the scientific method in action.
You will come away from this tutorial with increased confidence in the rigor of your research and the fundamental knowledge to design scientifically sound experiments.
Watch the full presentation here: https://events.bitesizebio.com/confirmation-bias-and-the-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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For many researchers, modern science has become about getting results rather than asking questions. Results mean publications, and publications mean more funding.
However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true or false, and objectively interpreting the results.
Nonetheless, the results-focused attitude to doing science is widespread, and it comes with a huge drawback—it encourages confirmation bias—making us prone to searching for evidence and interpreting findings to support proposed answers we believe to be true.
This episode of Listen In explores what confirmation bias is and how to avoid it.
Learn why confirmation bias is so common, how it creeps into our thinking, and how it degrades the quality of our experimental designs and findings.
You will come away from this tutorial with the tools needed to defend against confirmation bias, challenge what you believe, and reorient yourself from a results-focused outlook to a method-focused one.
Watch the full presentation here: https://events.bitesizebio.com/confirmation-bias-and-the/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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